Lu Chuang detection program: Determination of propionic acid additives in soy products by gas chromatography

In recent years, with the rapid development of the food industry, excessive or illegal use of food preservatives in food processing has been repeatedly prohibited. Propionic acid and its salts are one of the commonly used preservatives in food processing at home and abroad. They are widely used in cakes, raw grains, soy sauce, noodles and soy products. However, the consumption of excessive propionate will pose a threat to the safety of consumers, and the amount of food added should be analyzed and controlled to strictly control the amount of use.

Detection of propionic acid additives in soy products: GC method

According to the relevant provisions of the "Sanitary Standards for the Use of Food Additives" (GB 2760-2011), the limit of propionic acid and its sodium and calcium salts (calculated as propionic acid) in raw and moist noodles is 0.25 g/kg, in the original grain. The limit is 1.80 g/kg and the limit is 2.50 g/kg in legumes, bread, pastries, vinegar and soy sauce.

At present, the determination methods of propionic acid additives in foods mainly include gas chromatography, liquid chromatography, gas chromatography-mass spectrometry, and ion chromatography. The method of GB/T 5009.120-2003 mainly extracts propionic acid in the sample by steam distillation, and then uses gas chromatograph to measure. It has simple operation and few external interference factors, and can be popularized and applied quickly.

Methods Required instruments: gas chromatograph ; vortex shaker; ultrapure water meter; ultrasonic cleaner; low speed centrifuge.

Operating conditions: column (120m × 250um × 0.25um); detector: FID (hydrogen flame ionization detector); heater: 300 ° C; air flow: 400mL / min; hydrogen flow: 30mL / min; Flow rate (nitrogen): 25 mL/min; carrier gas: hydrogen, flow rate: 1.5 mL/min; programmed temperature: 50 ° C for 1 min, 20 ° C / min to 230 ° C, 230 ° C after 10 min;

       Determination method: Weigh 2.0 g of homogenized sample in 50 ml stoppered plastic centrifuge tube, add 1 uL/mL sulfuric acid 1 mL , add ultra-pure water 10 mL , add ethyl acetate 10 mL , seal, and vortex The mixture was vortexed and shaken, and after sonication for 10 min , centrifuged ( 8000 rpm ) for 3 min in a centrifuge , and 1 mL of the supernatant was taken in the injection vial for testing.  

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What Do You Know About The Effects Of Organic Fertilizer?

Biological organic fertilizer refers to the active microorganisms compounded by specific microorganisms and organic materials from animal and plant residues after the harmless treatment and decomposition, it also contains nitrogen, phosphorus and potassium fertilizers with rich organic matters. It has many advantages of no pollution and durable effects, it is also helpful in strengthening seedlings, ameliorating soil, increasing yields and improving qualities. Meanwhile, using organic fertilizer can avoid environmental pollution, ecological destruction, fertility decline and other drawbacks caused by chemical fertilizers and pesticides.

The specific functional microorganism of biological organic fertilizer

➤ To cultivate a large number of azotobacter by using carbon source and nitrogen from the organic matters in soil and the nitrogen dioxide in the air.

➤ To decompose phosphate-solubilizing bacteria and potassium bacteria with P and K in the soil or the soil parent material.

➤ To inhibit pathogen growth and promote the reproduction of bacteria.

 

Generally, the bio organic fertilizer has quantitative microbes per gram, after applied into soil, under the condition of 25~35℃ of ground temperature, pH 5.5 – 8.5, 50 ~ 70% of water in the soil, no fungicide residues, the azotobacter, phosphate-solubilizing bacteria, potassium bacteria and bacteria can breed a generation every 20 minutes with growing exponentially. Each beneficial bacteria is a storehouse of fertilizer, the production factory of beneficial bacteria is moved under ground and produces nitrogen, phosphorus, potassium and other trace elements for plants to absorb, thus increasing the yield and quality, improving the quality of soil, promoting cultivating and reducing the occurrence of pests and diseases.

EU organic food certification:

Category

SO2

Pesticide

Organic goji berry

<10ppm

No pesticide (with organic certificate)

· USDA organic Certificate No.: A2015–00494/2016-07387 Authority :Kiwa BCS 

· EU organic Certificate No.: A2015-00494/2016-07386 code:CN-BIO-141 Authority :Kiwa BCS



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