Phytosterol is an active ingredient widely distributed in the roots, stems, leaves and fruits of plants, such as various vegetable oils and nuts. It is a general term for sterols with similar structural and biochemical properties to cholesterol. The most common ones include beta-sitosterol, campesterol, stigmasterol, followed by saturated forms of phytosterols, namely beta-sitosterol and campesterol. This "fat-like" cannot be synthesized in the human body, the only way is through dietary intake. It is widely used in medicine, industry, daily chemical, agriculture, and is a multi-purpose and pure natural substance, which is regarded as the "key of life" by scientists.

Natural plant sterols have important physiological activities for the human body, can inhibit the absorption of cholesterol by the human body, promote the degradation and metabolism of cholesterol, inhibit the biochemical synthesis of cholesterol, and have significant prevention for coronary heart disease, atherosclerosis, ulcers, skin squamatization, etc. And the therapeutic effect, has a strong anti-inflammatory effect, and can also act as a preventive agent for the formation of gallstones. In addition, the high level of phytosterols is also one of the important indicators for judging the quality of various vegetable oils. In view of the important role of phytosterols in various fields, Lu Chuang analysis according to GB/T25223-2010/ISO 12228-1999 "the determination of the sterol composition of animal and vegetable oils and the determination of total sterol gas chromatography" (International Organization for Standardization ISO also uses this method As a standard method for the analysis of plant sterols, optimized sample pretreatment method and gas chromatograph FID capillary analysis test conditions, qualitative and quantitative analysis of plant sterols, the separation effect of each sterol is good, and the number of peaks is less; The result has the advantages of accurate and reliable analysis, simple operation and less external interference.