I. Product Description
TC-EM-1 is a polymeric emulsifier, which is a low molecular weight, high electropositive organic polymer. It has followed advantages:
1. Easy to use, do not need cooking. Reduce cooking equipment for solid starch and lower down technical condition fluctuation for cooking the starch.
2. Use current water to dilute and prepare, without heating.
3. Provide a strong positive charge to the system
4. High emulsion stability
II. Product Feature
Appearance
Red brown liquid
Solid content %
25. 50±0.5
Ionicity
Cation
PH value
3.50~4.50
Viscosity(mpa.s/25℃)
60.0
III. Product Usage Safety Instruction:
TC-EM-1 may be stimulative to skin and eyes, and it is recommended to use gloves and protective glasses when use. If eye or skin comes into direct contact with this product, immediately rinse with plenty of water for at least 15 minutes.
IV. Application
As a emulsifier and stabilizer of AKD,TC-EM-1 can provide a good stability of the emulsion. With the mechanical stirring, it can disperse AKD as required in the production process, which average particle diameter more than 80% can be less than 1.2 microns. It can also maintain a relative long time after the emulsification, and it has a certain electrical neutral to the slurry.
Please note, under high hardness, the hydrolysis rate of AKD emulsion will be greatly improved. So in order to reduce the rate of hydrolysis, the PH value of emulsified should be controlled between 4.5~5.0 by Citric Acid. TC-EM-1 has low PH value, which saves this part of the operation.
For certain application, the usage of TC-EM-1 should also follow the operating instructions of our company.
V. Other Information
Package: 1000Kg /IBC barrel
Storage: in cool and dry place, avoid frozen and sunshine
Shelf life: 6 months.
AKD Emulsifier,Starch Based Emulsifier,AKD Emulsifying Agent,25% AKD Emulsifier Shandong Tiancheng Chemical Co., Ltd. , https://www.tianchengchemical.com
Rat Bradykinin ELISA Kit Instructions
Shanghai Xitang Biotechnology Co., Ltd. 021-55229872, 65333639
Rat Bradykinin ELISA Kit
 ( used in serum, plasma, cell culture supernatants and other biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-rat Bradykinin mAb was coated on the microtiter plate, the Bradykinin in the standard and the sample was combined with the mAb, and biotinylated anti-rat Bradykinin was added to form an immune complex attached to the plate, horseradish peroxidation. The enzyme-labeled Streptavidin is combined with biotin, and the substrate working solution is blue. Finally, the stop solution sulfuric acid is added, and the OD value is measured at 450 nm. The Bradykinin concentration is directly proportional to the OD value, and the Bradykinin can be obtained by drawing a standard curve. concentration.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 200ng/bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collection of specimens: serum, plasma (EDTA, citrate, heparin anticoagulation), cell culture supernatant, tissue homogenate, etc., as early as possible, stored at 2-8 ° C for 48 hours; longer time must be frozen (-20 °C or -70 °C) Save to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 1 ml of distilled water before use and mix well to prepare a 200 ng/ml solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 100 ul of 200 ng/ml standard solution to the first tube, mix and aspirate 500 ul with a pipette, and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution per well, set 37 The reaction was carried out in the dark at °C for 15 minutes.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Using standard products 20, 10, 5, 2.5, 1.25, 0.625, 0.312, 0 ng/ml as the abscissa and OD as the ordinate, plot on the coordinate paper and draw the standard curve.
3. Find the corresponding Bradykinin content on the graph based on the OD value of the sample.
Kit performance
1. Sensitivity: The minimum Bradykinin detection concentration is less than 0.2 ng/ml.
2. Specificity: Recombinant or natural rat Bradykinin can be detected simultaneously. Does not cross-react with other cytokines in rats.
3. Repeatability: The coefficient of variation in both the plate and the plate is less than 10%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4 o C refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!