Garlic is planted all over the world, but China has always
been the largest producer of garlic, with about 10.5 million tons of garlic
annually, accounting for more than 77% of world output. Jinxiang is the largest
garlic planting and storing area in the world. Except jinxiang, the origin of
garlic in China also include pizhou city ,Jiangsu province; laiwu city, Shandong
province; linyi city, Shandong province; shanghe town ,jinan city, Kaifeng
city, Henan province; pengzhou city, Sichuan province; haozhou city, Anwei
province,Xinjiang province and so on. The garlic from jinxiang is the most
popular with its high quality in the world.If you are insistently pursuing the
quality and taste, the garlic from jinxiang will be your best choice.Except Fresh Garlic ,we also supplied Fresh Ginger , Air Dried Ginger , Fresh Onion , Peeled Onion , Fresh Chestnut , Sweet Corn , Fresh Taro , Fresh Potato , Fresh Baby Mandarin, Fresh Red Grape, Fresh Pomelo , Fresh Ya Pear Chaotian Chili , Paprika Powder , Fresh Apple and so on.
Name
Normal white garlic
Size
4.5cm,5.0cm, 5.5cm, 6.0cm, 6.5cm,7cm and up.
Place of origin
Jinxiang, JiNing, Shandong, China
Edibleness
Reducing
bacteria, keeping the heart in good condition and immunity.
Certification
SGS;GAP
Supply period
The whole year
Fresh season: early June to mid September
Cold storage season: September to next June
Standard
Class A(suitable to export)
Packing
Loose Packing:
5/6/6.5/7/8/9/10/20KG MESH
BAG;
120Gx40/4.8KG CTN, 160G*40/6.4KG
CTN, 200gx20/4kg
CTN with pallet, 240G*42/10KG CTN , 300G*34/10KG CTN ,400Gx20/8KG CTN, 500Gx20/10KG CARTON, 900Gx10/CTN,1KGx10/10KG CTN,
4P
200G*12/2.4KG BAG,250Gx20/5KG BAG , 300Gx8/2.4 BAG ,
500gx10/5kg
MESH BAG,10P 100G*10/8KG BAG,
Quantity/conveyance
26/27/28/MTS
FOR ONE 40RH CONTAINER
Shipment
degree
-3°- 0°C
Delivery time
5-7 days after got buyer's deposit
Shipment port
Qingdao Port of China
Main importing
Country
UK, Greece, Poland, Russia, France, Norway, Turkey, U.A.E, Kuwait,Algiers, Arabia, Bangladesh, Thailand, Malaysia, Brazil, Canada, America
Normal White Garlic 6.0-6.5Cm,Fresh White Garlic,Natural Fresh White Garlic,6.0Cm Normal White Garlic Jining Fuyuan Fruits And Vegetables Co., Ltd. , http://www.fuyuanfv.com
First, commonly used gene cloning technology
1, through the analysis and identification of known gene products This technology is mainly through biochemical and pathological studies to identify the protein product of the relevant genes, and analyze the amino acid sequence of the protein, infer the gene sequence encoding the protein, and then through the antibody , oligonucleotide probes or PCR-produced probes screen the library to isolate the gene of interest. For example, the Bacillus thuringiensis insecticidal crystal protein gene (Bt gene), cowpea trypsin inhibitor gene (CpTI gene), and viral coat protein gene (CP gene) commonly used in plant pest resistance gene engineering. When similar genes of other plants have been isolated and the nucleotide sequences are highly conserved, these known gene fragments can also be directly used as probes to screen plant gene libraries that have not been cloned into the gene and can also be isolated. Unknown new gene.
2, by genetic phenotyping (1) genetic marker method. The method uses transposons or T-DNA inserted into the genome of a plant to cause the inactivation of a certain gene to produce some mutants, and then use the corresponding transposon or T-DNA to screen the mutant library to select the positive clones. Fragments are probes, and wild type plants are screened for gene isolation by library. For example, if a plant with a functional transposition factor system is crossed with another genetically different plant of the same species, screening in the hybrid progeny results in phenotypic disruption due to insertion of a transposition factor into a specific gene sequence. Alternatively, the mutant strain is modified to construct a gene library using the homozygous mutant strain, and the transposition factor is then labeled with an isotope label as a probe, and a target gene with an homologous translocation factor is selected from the library. This method is mainly limited to diploid self-pollination crops such as corn and snapdragon. Viviparious-1 gene related to maize seed development and some genes related to flower development of snapdragon have been isolated by this method.
(2) The elicitor's host receptor gene cloning technology. The technique uses an incompatible interaction between the elastase-encoded elastase gene and the receptor encoded by the host resistance gene, and uses the pathogen elicitor protein as a clue to isolate and clone some chitin. Disease resistant genes, such as the resistance gene cf9 corresponding to avr9 in tomato and the avirulence gene, the resistance gene pto corresponding to avrPto, and the resistance gene rpS2 corresponding to avrbrs2 in Arabidopsis thaliana.
3. Mapping-based cloning techniques Mapping-based cloning techniques have broad application prospects for the isolation of genes of unknown products. The basic premise of this method is gene localization. Then it begins with close-linked molecular markers such as RFLP, etc., and gradually approaches the target gene through chromosome walking to finally clone the gene. Its main steps include:
(1) Targeting the target gene on a high density molecular marker linkage group;
(2) Using PFGE to convert the genetic map distance of the concatenated marker into a physical distance;
(3) Construct YAC library, find the YAC clone with linkage markers, and obtain the DNA fragment of the target gene through the sequencing of clones;
(4) Confirm the DNA fragment where the gene is located by transformation and functional complementation tests. If this technology has been used to isolate the tomato anti-root-knot nematode mi gene and Arabidopsis bacteriophage resistant RPM1 gene and so on.
Second, the development of new gene cloning technology
1. To isolate the gene by studying the phenotype of the deletion mutant (1) Nuclear DNA subtractive method. This method mainly uses the difference of only 1-2 DNA fragments between many deletion mutants and their non-deleted homologous parent. By mixing a large amount of specially labeled mutant DNA with a small amount of non-deleted parental DNA, the denaturation is performed at an appropriate temperature. Refolding, after the single-stranded DNA in the reaction system is paired into double strands, the labeled mutant DNA and the parental DNA paired with it are removed. After several rounds of selection, only the parent DNA that was deleted in the mutant nuclear DNA is finally left in the reaction system, and the parental specific DNA fragment is finally cloned.
(2) Nuclear DNA ablation - PCR method. This method is the development of the nuclear DNA depletion method. It combines the non-deleted parental DNA (prepared DNA) treated with specific restriction enzymes with a large number of ultrasonically-cleaved, photobiotin-labeled mutant DNA (subtracted probe DNA). Mix, denature, and add avidin-coated multi-polyethylene globules to remove various hybrids. Enrich the parental DNA and add the adapter sequence and T4 DNA ligase after several rounds of selection. Single linker sequences were used as PCR primers for PCR amplification. Finally, PCR products were directly cloned or labeled with 32P and used as probes to hybridize with parental DNA or nuclear DNA libraries to determine the genes encoded by these DNA fragments. Gene gal associated with gibberellin synthesis and salt stress-inducing genes of Arabidopsis thaliana.
2. Direct isolation of coding sequences from large genomic regions Only a small portion of eukaryotic genomic DNA encodes mRNA, and most of them are introns, exons, and repeats. Currently, large-scale genomic sequencing is used to find and clone genes. The new gene is not yet feasible. In some methods for the isolation of expression sequences, cDNA capture has been a successful method and has been used for the location cloning of many genes.
(l) cDNA library differential screening method, which is a method of directly separating tissue or developmentally-expressed genes. For example, for two different tissue cells, first extract their poly(A)+ mRNA and construct these two Tissue cDNA libraries, then using these mRNAs as templates to synthesize radiolabeled cDNA probes, and using these two probes to hybridize with two sets of replicates of a single cDNA library, respectively, and clones that hybridize with both probes. Genes that are expressed in both tissue cells, and clones that hybridize to only one probe are mRNAs that are specifically expressed in a tissue cell. These clones can then be sequenced, compared, and identified, and can be isolated. A tissue-specific gene. In this way, genes specifically expressed in roots, leaves, and reproductive organs have been isolated.
(2) cDNA capture method. The cDNA capture method is an expression-based gene separation technique. It directly captures cDNA fragments using genomic DNA such as yeast artificial chromosomes (YACs) to rapidly isolate expression sequences from large genomic regions. The main technique is to use PCR to amplify mixed cDNA pools or cloned cDNA libraries from different tissues. The amplified cDNA fragments hybridize with the genomic target DNA that was randomly labeled with biotin, and capture the target on the avidin labeled magnetic beads. The cDNA fragments are hybridized with DNA, and then the cDNA captured on the magnets is eluted and PCR amplified. Then a second round of screening is performed, and the selected cDNA fragments are cloned into the vector.
3. Screening for cloned genes by studying mRNA differential expression
In 1992, Zeng Peng et al established mRNA differential display reverse transcription PCR (DDRT-PCR) based on mRNA differential subtractive hybridization, which is a general method for mRNA differential expression. Due to the defects and deficiencies of the technology, many studies have been conducted. The technique has been improved and optimized by primer design, Northern hybridization and gel sequencing, and many new techniques have been developed, such as random amplified PCR amplification of RNA fingerprinting (RAP) and sequential differential display (ODD). ), Genome Difference Display (GDD), RC4D (RFLP-Coupled domain-direded DD), etc.
Differential display of mRNA has been widely used in the cloning of differentially expressed genes in developmental and physiological processes, and is particularly suitable for comparison and screening between several parallel materials. The RC4D method can be used to analyze the differential expression of different members of a particular multigene family in a tissue during plant development, such as the differential expression of MAD-box genes in six male and female inflorescences that have been isolated from maize inbreds, from wheat and The HSP family genes were isolated from Arabidopsis thaliana.
4. Phenotypic Cloning The phenotypic cloning method uses mRNA as the starting material, uses RT-PCR and linker technology to recover the differentially displayed gene bands and uses them as probes to scan and screen new cDNA or genomic DNA libraries. The genes. The latest technologies developed in this area include: cDNA differential analysis (RDA), tag adapter PCR (ATAC-PCR), suppression subtractive hybridization (SSH), continuous gene expression analysis (SAGE), and cDNA 3-terminal restriction Fragmented fragment display (RFD), etc. These techniques eliminate the tedious procedures of molecular marker mapping and simultaneously isolate differentially expressed genes from multiple unknown products.
5. Use DNA chip technology to screen new genes
DNA chip technology uses the principle of nucleic acid hybridization to detect unknown molecules. It is composed of nucleic acid fragments, such as a series of oligonucleotide probes labeled with specific fluorescence, immobilized on slides or nylon membranes in a pre-designed arrangement to form bio-integrated membranes, with different labeled free target molecules (DNA or RNA). Hybridization, or hybridization of different target molecules on a biointegrated membrane to free probes, followed by detection of hybridization signals using fluorescent signal detectors and processors to detect the different wavelengths of light emitted by hybrid or unhybridized molecules. If fully hybridized, a strong fluorescence signal or a special wavelength signal is emitted, and the incomplete hybridization signal is weak. If the hybridization is not possible, no fluorescence signal is detected or only the original fluorescence signal on the chip is detected. These different regions of the fluorescence signal on the chip composition of the fluorescence spectrum, can be excited and detected by laser confocal microscopy, computer software to detect the DNA sequence and its changes. For example, from Stanford University, a PCR amplification method was used to amplify the inserted gene from a peripheral blood lymphocyte cDNA library, and 1046 amplification products of unknown sequences were obtained. These amplification products were spotted onto coated slides to prepare a DNA chip. After treatment with SDS, NaBH4, etc., and after thermal denaturation at 95°C, they were hybridized with heat shocked T cells and untreated T cells, respectively. Seventeen differentially expressed genes were found by laser scanning confocal scanning system, 11 of which were Heat-induced, the remaining six were heat-suppressed, and sequence analysis revealed that three of them were undiscovered new genes. Recently, a cDNA chip has been used on plants to detect the gene expression of strawberry and petunia.
DNA chip technology is a very useful technology for discovering new genes and analyzing the expression of various genes in different time and space. Some scholars have prospected their applications in agriculture, such as the application of DNA chip technology is expected to learn more about plant growth. The intrinsic mechanism of development, the mechanism of action of plant growth hormones, rapid laboratory analysis of transgenic plants, understanding of the effects of small molecules on gene expression, and acceleration of DNA polymorphism detection.
In summary, the various plant unknown gene isolation and cloning techniques mentioned above have been applied to research and analysis. The research and analysis objects have their own advantages and disadvantages, and some have been applied to the research and development of plant genetic resources, and some are gradually applied to plants. . There is no doubt that the development of these plant gene separation technologies has great potential for application in plant molecular biology and genetic engineering. With the continuous discovery of new genetic resources, many crop traits such as yield, quality, resistance, Plant morphology, maturity, the development of floral development, as well as some complex physiological and biochemical traits are expected to be further improved.
5/6/6.5/7/8/9/10KG CARTON
Small Packing:
Plant Gene Cloning Technology and Its Development
A gene is a genetic unit with a certain locus on a chromosome and is a nucleotide sequence of a certain length in a DNA molecule. The growth and development of plants is a comprehensive phenomenon of gene expression in space-time based on a variety of metabolic and physiological processes. The development and isolation of potential various valuable genes and in-depth study of its expression mechanism have improved the variety of crops. Significance. Therefore, the cloning of plant genes and the development of related technologies have caused people's increasing attention and investment. In recent years, the research methods have been continuously improved and new technologies have emerged continuously. This will further study such as various genes regulating plant growth and development, and adversity. Genes such as defense-responsive genes, plant apoptotic genes, and others provide new ways.