Application of colloidal gold rapid diagnostic reagent in the detection of clenbuterol: Clenbuterol is a type of animal drug that acts to reduce fat and increase lean meat. At present, clenbuterol is mainly clenbuterol in China, which belongs to β-agonist drugs. Clenbuterol has very dangerous side effects, which can lead to arrhythmia and heart attacks. Colloidal gold particles of different sizes can be prepared according to different reducing agents. The methods commonly used to prepare colloidal gold particles are as follows: The importance of pure water in the production of colloidal gold : Effect of different gold particle size on solution color 2. Organic matter, in addition to possibly changing the pH, forms a combination with proteins. Or unstable, easy to inactivate, or affect the sensitivity of the probe. Preparation of interfering protein-gold complexes. Good colloidal gold turbid colloidal gold Microorganisms produce metabolites, and cell lysis also produces large amounts of impurities when the microorganisms die. Bringing various impurities such as particles, organic matter, ions, etc., affecting colloidal gold from various aspects.
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At present, the techniques for detecting clenbuterol mainly include colloidal gold method, high performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry. Wherein the colloidal gold immune chromatography method for its fast, simple, convenient, etc., is increasingly becoming an important method.
GICA method (Immune colloidal gold technique) is applied to an antigen-antibody colloidal gold as an immune marker tracer labeling technique. Colloidal gold is polymerized into a certain size of gold particles by chloroauric acid (HAuCl4) under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, citric acid, etc., and becomes a stable colloidal state due to electrostatic action.
White phosphorus reduction
2. Trisodium citrate reduction method.
3. Citrate-sodium citrate reduction method.
1. Ionic impurities in water change pH and ionic strength. The pH has a certain effect on the final particle size. The color of the solution is related to its pH. The ionic strength affects the successful combination of colloidal gold and protein, or leads to the aggregation of colloidal gold particles and reduces the detection ability of colloidal gold.
3. The particles interfere with the formation of colloidal gold particles, and the particles formed are of different sizes, and the color is reddish, colorless or opaque.
Therefore, the preparation of colloidal gold must use ultra-pure water with quality assurance. Ions must be used to remove ions, activated carbon and UV lamps to remove organic matter, membrane filtration to remove particles and microorganisms, and the like.