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96-channel pipette in the biotechnology industry application method set
96-channel pipette in the biotechnology industry application method set
In the biotech industry, more and more applications or developments need to be placed on 96-well plates to increase the number of tests or to increase the parallelism of the reaction. The 96-channel pipette can be used properly to take full advantage of the 96-well plate.
First, high-throughput nucleic acid extraction
Nucleic acid extraction is perhaps the most fundamental and important task in modern biology. Disease diagnosis, genetic breeding, drug screening, developmental expression, molecular evolution... Almost every part of the biological sciences is inseparable from the nucleic acid components required for isolation and purification. Moreover, many studies still need to obtain reliable data through an increase in the number.
For nucleic acid extraction equipment, the requirements for it are reliable, reproducible, and low in operating costs. However, most of the automated nucleic acid extraction equipment currently on the market either have insufficient processing throughput, or the operating cost is too high, or the cost performance is too low, and most researchers are not really satisfied.
The 96-channel pipette is used for parallel extraction of 96 samples, which greatly saves working time and improves the quality of extracted products. The results are reliable and consistent.
The use of 96-channel pipettes, combined with other auxiliary equipment for nucleic acid extraction, open reagents, truly high throughput, and the most cost-effective, is the savvy choice of researchers. It is especially suitable for research topics such as epidemiology, transgenic breeding, and biomarker screening.
Second, drug orthogonal screening
The use of orthogonal methods for the screening of the most effective working concentrations of various drugs often requires a lot of effort to configure the solution, which is very cumbersome and error-prone, including the addition of wrong holes or the addition of errors. The use of a 96-channel pipette saves working time and improves the quality of the combined configuration for more reproducible results.
Orthogonal screening of two drugs:
Simply configure a series of concentration gradients for each solution and pipette twice to configure 96 different combinations!
8 * 12 = 96, 2 pipetting
Orthogonal screening of three drugs:
Only need to configure a series of concentration gradients for each solution, 12 pipetting, can be configured into 4 * 96 different combination solutions!
8 * 12 * 4 = 384, 12 pipettings (3 pipettings into 1 dish) for 6 minutes.
Using a 96-channel pipette, it takes only 20 minutes (including the time to serially dilute 3 drugs), and it is easy to complete the configuration of 384 drug formula combinations. The whole process is controlled by itself, reliable and reliable, eliminating the need to operate the automatic pipetting platform. Kind of programming operation and worry.
Third, cell viability detection
The use of cells as a model for drug screening is already a routine means of drug development, whether it is pharmacodynamic studies or toxicological studies.
Research, are very dependent on cell viability test.
Standard cell viability test methods are based on 96-well plates. There is a relatively high requirement for the amount of cell plating, the amount of various reagents added, and the consistency of the reaction. The introduction of a 96-channel pipette makes this test more reproducible and results more reliable, reducing the operator's work intensity.
This method has been widely used in the detection of activity of some biologically active factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity measurement, etc., and is a widely accepted test method. The main error is derived from the amount of MTT and DMSO added, and the difference in reaction time. High-throughput pipettes can effectively eliminate these differences, so that the experimental data truly reflects the impact of drugs on cells, and this method will certainly make this method better serve the research of life sciences, especially drug development.
4. High-throughput screening of fermentation strains
Screening for the expression ability of the target product of genetically engineered or induced mutant engineering bacteria is a necessary means for obtaining highly expressed and genetically stable process species.
Traditional strain screening is based on culture plates, and monoclonal strains can be obtained. However, the screening of the fermentation expression can only be carried out with a flask, and then the content of the specific substance in the fermentation broth or the biological activity of the target substance is measured, and the later work is very large.
It has been reported that it is feasible to culture monoclonal spores with 96 deep well plates (Lollis et al., 2010). Moreover, for the detection of fermentation products, if a high-throughput method for measuring the whole plate with a 96-well plate can be established, the time for quantitative or qualitative measurement of the product can be greatly saved, achieving high work efficiency (Qi Xizhen et al., 2011). ; Zong Shasha et al., 2011)
Compared with the traditional shake flask screening method, the method is simple and convenient to operate, saves a lot of reagents and laboratory space, shortens the strain screening cycle, and increases the strain. The number of primary screenings can be high-efficiency, low-cost, and rapid screening of spinosad-producing strains (Lorris et al., 2010).
High-throughput screening technology has just started in China. But this technology must continue to be miniaturized, micro-scaled, cheaped and automated.
The development of direction is the main trend of future high-throughput screening research in China.
5. The coating of the ELISA plate
For bioreagent companies, the coating of the ELISA plate is the most critical part of controlling product quality. Between the hole and the hole, the plate must be kept at a high level of consistency to reflect the stability of the product. In addition, the workload of production is particularly large, and it is common to require thousands of boards per day.
Current working methods:
The traditional skilled workers cooperate with the multi-channel pipette combination mode, and there are personnel stability, equipment differences, and work intensity.
The problem has become a bottleneck in improving product reliability and expanding production capacity.
High-input solution:
The use of fully automatic production equipment will undoubtedly overcome the problems of the current mode, but there are also excessive input costs and commissioning time.
Long, production process reconfiguration, downtime, regular maintenance and other issues are not suitable for all bio-reagent companies.
The most cost-effective solution:
A more effective way is to improve the individual combat capability, so that each operator can do more work in the same working time.
Quantity, and actually reduce the intensity of its work. This requires an improvement in the production tool, which is the pipette.
Spray accuracy (150ul, one suction 6 spray):
Difference between boards: the average value of the whole board, the difference between the boards is within 2%;
Intraplate difference: 20 holes are taken at random, and the relative standard deviation is RSD < 1%.
Working speed (150ul, one suction 6 spray):
Theoretical speed (infinite solution, no need to organize the tray): 1 minute to complete a suction 6 spray
Actual speed (adding solution, stacking target disk): 250 disks in 1 hour and 1000 disks in 4 hours in 1 day.
96-channel pipette with piston pipetting, no need to adjust the volume according to the viscosity and temperature of the liquid
Trivial operation, there is no need to worry about the deviation or error of the needle tube blockage, the dead body is small, the maintenance cost is low, it is the pursuit
Reliable and stable production equipment of choice for various biological reagent manufacturers.
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