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Edible fungi strain preservation
The two most commonly used methods for preserving microbial cultures are cryogenic cryopreservation and liquid paraffin preservation. The first method, cryogenic preservation, is the simplest and most widely applied technique. It involves storing a growth-free, uncontaminated slant tube in a refrigerator at a temperature between 4°C and 6°C. This low-temperature environment slows down metabolic activity, allowing the culture to remain viable for an extended period. To maintain viability, the culture should be transferred to fresh slant medium every 2 to 3 months. This process ensures that the microorganism remains active and can be successfully cultivated when needed.
The second method, liquid paraffin preservation, is particularly useful for maintaining bacterial and fungal strains. In this technique, sterile liquid paraffin is carefully added to the top of the slanted agar in a sterilized test tube. The paraffin layer should cover the surface by about 1 cm to create an oxygen-free environment, which helps prevent contamination and reduces metabolic activity. After adding the paraffin, the tube is sealed with a cork or rubber stopper and then wax is applied around the seal to ensure airtight closure. Once properly sealed, the tubes are stored upright in a dry, cool indoor location. This method can preserve cultures for over one year, making it ideal for long-term storage when refrigeration is not available.
Both methods have their advantages and are chosen based on the type of microorganism, available resources, and storage conditions. Regular monitoring and periodic subculturing are essential to ensure the continued viability of preserved cultures.